Ectopic transcription due to inappropriately inherited histone methylation may interfere with the ongoing function of terminally differentiated cells

Rodriguez, J. D.; Reeves, M.; Wang, H.-L.; Chavez, J.; Rastogi, R.; Chavez, S.; Chadha, M.; Hill, E. J.; Corces, V. G.; Schmeichel, K.; Katz, D. J.

doi:10.1101/2023.10.29.564525

bioRxiv · 10.1101/2023.10.29.564525

Ectopic transcription due to inappropriately inherited histone methylation may interfere with the ongoing function of terminally differentiated cells

Rodriguez, J. D.·Reeves, M.·Wang, H.-L.·Chavez, J.·Rastogi, R.·Chavez, S.·Chadha, M.·Hill, E. J.·Corces, V. G.·Schmeichel, K.·Katz, D. J.

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Abstract

How mutations in histone modifying enzymes lead to neurodevelopmental disorders is unknown. We took advantage of the invariant embryonic lineage and adult nervous system in C. elegans to investigate a double mutant between spr-5/Lsd1/Kdm1a (H3K4me1/2 demethylase) and met-2/Setdb1 (H3K9 methyltransferase). We demonstrate that spr-5; met-2 double mutant worms have a severe chemotaxis defect caused by the ectopic expression of germline genes in somatic tissues. Despite this behavioral defect, we observe few embryonic lineage alterations and an intact adult nervous system. This raises the possibility that the abnormal chemotaxis behavior may be due to ongoing defects in terminally differentiated cells rather than alterations in development. Remarkably, we found that shutting off the ectopic germline expression rescues normal chemotaxis in the same spr-5; met-2 adult worms that had a chemotaxis defect earlier. This suggests that ongoing inappropriate transcription can block normal behavior in an intact nervous system. Based on these data, it is possible that the intellectual disability and altered behavior observed in human neurodevelopmental syndromes caused by mutations in histone modifying enzymes could be due to ongoing ectopic transcription and may be reversible.

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Rodriguez, J. D., Reeves, M., Wang, H.-L., Chavez, J., Rastogi, R., Chavez, S., Chadha, M., Hill, E. J., Corces, V. G., Schmeichel, K., Katz, D. J.. 2023-11-01. Ectopic transcription due to inappropriately inherited histone methylation may interfere with the ongoing function of terminally differentiated cells. https://doi.org/10.1101/2023.10.29.564525

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