Lack of chromokinesin Klp-19 creates a more rigid midzone and affects force transmission during anaphase in C. elegans

Zimyanin, V.; Magaj, M. M.; Yu, C.-H.; Gibney, T. V.; Mustafa, B.; Horton, X.; Siller, K.; Cueff, L.; Bouvrais, H.; Pecreaux, J.; Needleman, D.; Redemann, S.

doi:10.1101/2023.10.26.564275

bioRxiv · 10.1101/2023.10.26.564275

Lack of chromokinesin Klp-19 creates a more rigid midzone and affects force transmission during anaphase in C. elegans

Zimyanin, V.·Magaj, M. M.·Yu, C.-H.·Gibney, T. V.·Mustafa, B.·Horton, X.·Siller, K.·Cueff, L.·Bouvrais, H.·Pecreaux, J.·Needleman, D.·Redemann, S.

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Abstract

Recent studies have highlighted the significance of the spindle midzone, the region between the segregating chromosomes, in ensuring proper chromosome segregation. By combining 3D electron tomography, cutting-edge light microscopy and a novel single cell in vitro essay allowing single molecule tracking, we have discovered a previously unknown role of the regulation of microtubule dynamics within the spindle midzone of C. elegans by the chromokinesin KLP-19, and its relevance for proper spindle function. Using Fluorescence recovery after photobleaching and a combination of second harmonic generation and two-photon fluorescence microscopy, we found that the length of the antiparallel microtubule overlap zone in the spindle midzone is constant throughout anaphase, and independent of cortical pulling forces as well as the presence of the microtubule bundling protein SPD-1. Further investigations of SPD-1 and KLP-19 in C. elegans, the homologs of PRC1 and KIF4a, suggest that KLP-19 regulates the overlap length and functions independently of SPD-1. Our data shows that KLP-19 plays an active role in regulating the length of microtubules within the midzone as well as the size of the antiparallel overlap region throughout mitosis. Depletion of KLP-19 in mitosis leads to an increase in microtubule length and thus microtubule-based interactions in the spindle midzone, which affects spindle dynamics and force transmission. Our data shows that by localizing KLP-19 to the spindle midzone in anaphase microtubule dynamics can be locally controlled allowing the formation of a functional midzone. SummaryKLP-19 controls microtubule length in the spindle midzone of C. elegans, affecting spindle dynamics and force transmission during mitosis.

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Zimyanin, V., Magaj, M. M., Yu, C.-H., Gibney, T. V., Mustafa, B., Horton, X., Siller, K., Cueff, L., Bouvrais, H., Pecreaux, J., Needleman, D., Redemann, S.. 2023-10-26. Lack of chromokinesin Klp-19 creates a more rigid midzone and affects force transmission during anaphase in C. elegans. https://doi.org/10.1101/2023.10.26.564275

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