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bioRxiv · 10.1101/2023.10.26.564242

KIF1C activates and extends dynein movement through the FHF cargo adaptor

Ali, F. A.·Zwetsloot, A. J.·Stone, C. E.·Morgan, T. E.·Wademan, R. F.·Carter, A. P.·Straube, A.
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Abstract

Cellular cargos move bidirectionally on microtubules due to the presence of opposite polarity motors dynein and kinesin. Many studies show these motors are co-dependent, whereby one requires the activity of the other, although the mechanism is unknown. Here, using in vitro motility assays, we show that the kinesin-3 KIF1C acts both as an activator and a processivity factor for dynein. Activation only requires a fragment of the non-motor tail of KIF1C (KIF1C-stalk) to bind the cargo adaptor HOOK3. Cryo-EM, crosslinking mass spectrometry and AlphaFold2 predictions reveal this binding site to be separate from that of two constitutive factors (FTS and FHIP), which link HOOK3 to small G-proteins on cargos. We provide a structural model for how the FTS-HOOK3-FHIP1B (FHF) complex is auto-inhibited and explain how the KIF1C-stalk relieves this inhibition. Collectively, our work provides a molecular explanation for co-dependency by revealing that the mutual activation of dynein and kinesin is mediated through their shared adaptor. Many adaptors bind both dynein and kinesins, suggesting this mechanism could be generalised to other bidirectional complexes.

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Ali, F. A., Zwetsloot, A. J., Stone, C. E., Morgan, T. E., Wademan, R. F., Carter, A. P., Straube, A.. 2023-10-26. KIF1C activates and extends dynein movement through the FHF cargo adaptor. https://doi.org/10.1101/2023.10.26.564242

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