Tumor-infiltrating nerves functionally alter brain circuits and modulate behavior in a male mouse model of head-and-neck cancer

Barr, J.; Walz, A.; Restaino, A. C.; Williamson, C. S.; Amit, M.; Faber, S. M.; Vichaya, E. G.; Spanos, W. C.; Dantzer, R.; Talbot, S.; Vermeer, P. D.

doi:10.1101/2023.10.18.562990

bioRxiv · 10.1101/2023.10.18.562990

Tumor-infiltrating nerves functionally alter brain circuits and modulate behavior in a male mouse model of head-and-neck cancer

Barr, J.·Walz, A.·Restaino, A. C.·Williamson, C. S.·Amit, M.·Faber, S. M.·Vichaya, E. G.·Spanos, W. C.·Dantzer, R.·Talbot, S.·Vermeer, P. D.

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Abstract

Cancer patients often experience changes in mental health, prompting an exploration into whether nerves infiltrating tumors contribute to these alterations by impacting brain functions. Using a mouse model for head and neck cancer and neuronal tracing we show that tumor-infiltrating nerves connect to distinct brain areas. The activation of this neuronal circuitry altered behaviors (decreased nest-building, increased latency to eat a cookie, and reduced wheel running). Tumor-infiltrating nociceptor neurons exhibited heightened calcium activity and brain regions receiving these neural projections showed elevated Fos as well as increased calcium responses compared to non-tumor-bearing counterparts. The genetic elimination of nociceptor neurons decreased brain Fos expression and mitigated the behavioral alterations induced by the presence of the tumor. While analgesic treatment restored nesting and cookie test behaviors, it did not fully restore voluntary wheel running indicating that pain is not the exclusive driver of such behavioral shifts. Unraveling the interaction between the tumor, infiltrating nerves, and the brain is pivotal to developing targeted interventions to alleviate the mental health burdens associated with cancer. Significance StatementHead and neck cancers are infiltrated by sensory nerves which connect to a pre-existing circuit that includes areas in the brain. Neurons within this circuit are altered and mediate modifications in behavior.

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Barr, J., Walz, A., Restaino, A. C., Williamson, C. S., Amit, M., Faber, S. M., Vichaya, E. G., Spanos, W. C., Dantzer, R., Talbot, S., Vermeer, P. D.. 2023-10-20. Tumor-infiltrating nerves functionally alter brain circuits and modulate behavior in a male mouse model of head-and-neck cancer. https://doi.org/10.1101/2023.10.18.562990

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