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bioRxiv · 10.1101/2023.10.13.562314

FluxNorm: Toolbox for metabolic flux assay normalization by in situ cell counting

Djaja, N. A.·Bracha, T.·Yu, S. B.·Wang, H.·Carlson, N. M.·Pekkurnaz, G.
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Abstract

Plate-based quantitative metabolic flux analysis has emerged as the central technology to examine cellular metabolism and mitochondrial bioenergetics. However, accurate interpretation of metabolic activity between different experimental conditions in multi-well microplates requires data normalization based on in situ cell counts. Here, we describe FluxNorm, a platform-independent semi-automated computational workflow, validated for three different cell types, to normalize cell density for accurate assessment of cellular bioenergetics.

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Djaja, N. A., Bracha, T., Yu, S. B., Wang, H., Carlson, N. M., Pekkurnaz, G.. 2023-10-14. FluxNorm: Toolbox for metabolic flux assay normalization by in situ cell counting. https://doi.org/10.1101/2023.10.13.562314

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