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bioRxiv · 10.1101/2023.10.04.559423

Cross-Tissue Specificity of Pediatric DNA Methylation Associated with Cumulative Family Adversity

Chan, M. H.-M.·Merrill, S. M.·Fatima, F.·MacIsaac, J. L.·Obradovic, J.·Boyce, W. T.·Kobor, M. S.
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Abstract

BackgroundCumulative family adversity (cumulative FA), characterized by co-occurring stressors in a family context, may be biologically embedded through DNA methylation (DNAm) and contribute to later health outcomes. Materials & MethodsWe compared epigenome-wide DNAm associated with cumulative FA in buccal epithelial cells (BECs; n=218) and peripheral blood mononuclear cells (PBMCs; n=51) from 7-13-year-old children in Canada, accounting for sex, age, predicted cell-type proportion, and genetic ancestry. ResultsHigher levels of cumulative FA were associated with DNAm at seven sites, primarily in stress- and immune-related genes, only in PBMCs. Negative mother-child interaction contributed to this association. ConclusionsThe findings of this study suggested that PBMC DNAm can be used as a marker for biological embedding of cumulative FA.

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Chan, M. H.-M., Merrill, S. M., Fatima, F., MacIsaac, J. L., Obradovic, J., Boyce, W. T., Kobor, M. S.. 2023-10-06. Cross-Tissue Specificity of Pediatric DNA Methylation Associated with Cumulative Family Adversity. https://doi.org/10.1101/2023.10.04.559423

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