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bioRxiv · 10.1101/2023.10.03.560768

The G1/S transition is promoted by Rb degradation via the E3 ligase UBR5

Abstract

Mammalian cells make the decision to divide at the G1/S transition in response to diverse signals impinging on the retinoblastoma protein Rb, a cell cycle inhibitor and tumor suppressor. Rb is inhibited by two parallel pathways. In the canonical pathway, Cyclin D-Cdk4/6 kinase complexes phosphorylate and inactivate Rb. In the second, recently discovered pathway, Rbs concentration decreases during G1 to promote cells progressing through the G1/S transition. However, the mechanisms underlying this second pathway are unknown. Here, we found that Rbs concentration drop in G1 and recovery in S/G2 is controlled by phosphorylation-dependent protein degradation. In early G1 phase, un- and hypo-phosphorylated Rb is targeted by the E3 ligase UBR5. UBR5 knockout cells have higher Rb concentrations in early G1, exhibit a lower G1/S transition rate, and are more sensitive to Cdk4/6 inhibition. This last observation suggests that UBR5 inhibition can strengthen the efficacy of Cdk4/6 inhibitor-based cancer therapies.

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BibTeXRIS

Skotheim, J., ZHANG, S., Zatulovskiy, E., Valenzuela, L. F.. 2023-10-04. The G1/S transition is promoted by Rb degradation via the E3 ligase UBR5. https://doi.org/10.1101/2023.10.03.560768

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