Quantitative Assessment of Morphological Changes in Lipid Droplets and Lipid-Mito Interactions with Aging in Brown Adipose

Crabtree, A.; Neikirk, K.; Whiteside, A.; McKenzie, J.; Vue, Z.; Vang, L.; Owens, T. C.; Oliver, A.; Zeleke, F.; Beasley, H. K.; Garza-Lopez, E.; Scudese, E.; Koh, A.; Navarro, S.; Lam, J.; Kirk, B.; Mungai, M.; Sweetwyne, M. T.; Koh, H.-J.; Zaganjor, E.; Damo, S. M.; Gaddy, J. A.; Williams, C.; McReynolds, M.; Marshall, A. G.; Hinton, A.

doi:10.1101/2023.09.23.559135

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bioRxiv · 10.1101/2023.09.23.559135

Quantitative Assessment of Morphological Changes in Lipid Droplets and Lipid-Mito Interactions with Aging in Brown Adipose

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Abstract

The physical characteristics of brown adipose tissue (BAT) are defined by the presence of multilocular lipid droplets (LD) within the brown adipocytes and a high abundance of iron-containing mitochondria, which give it its characteristic color. Normal mitochondrial function is, in part, regulated by organelle-to-organelle contacts. Particularly, the contact sites that mediate mitochondria-LD interactions are thought to have various physiological roles, such as the synthesis and metabolism of lipids. Aging is associated with mitochondrial dysfunction, and previous studies show that there are changes in mitochondrial structure and proteins that modulate organelle contact sites. However, how mitochondria-LD interactions change with aging has yet to be fully clarified. Therefore, we sought to define age-related changes in LD morphology and mitochondria-lipid interactions in BAT. We examined the three-dimensional morphology of mitochondria and LDs in young (3-month) and aged (2-year) murine BAT using serial block face-scanning electron microscopy and the Amira program for segmentation, analysis, and quantification. Analysis showed reductions in LD volume, area, and perimeter in aged samples compared to young samples. Additionally, we observed changes in LD appearance and type in aged samples compared to young samples. Notably, we found differences in mitochondrial interactions with LDs, which could implicate that these contacts may be important for energetics in aging. Upon further investigation, we also found changes in mitochondrial and cristae structure for mitochondria interacting with LD lipids. Overall, these data define the nature of LD morphology and organelle-organelle contacts during aging and provide insight into LD contact site changes that interconnect biogerontology and mitochondrial functionality, metabolism, and bioactivity in aged BAT. O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=141 SRC="FIGDIR/small/559135v2_ufig1.gif" ALT="Figure 1"> View larger version (27K): org.highwire.dtl.DTLVardef@e5b7org.highwire.dtl.DTLVardef@e965e5org.highwire.dtl.DTLVardef@120c7e9org.highwire.dtl.DTLVardef@124bfb5_HPS_FORMAT_FIGEXP M_FIG Graphical Abstract Workflow highlighting the process of murine interscapular BAT extraction, processing and imaging serial block facing-scanning electron microscopy and using Amira software for 3D reconstruction of LDs and mitochondrial lipid contact sites to elucidate their structure across aging. C_FIG

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Crabtree, A., Neikirk, K., Whiteside, A., McKenzie, J., Vue, Z., Vang, L., Owens, T. C., Oliver, A., Zeleke, F., Beasley, H. K., Garza-Lopez, E., Scudese, E., Koh, A., Navarro, S., Lam, J., Kirk, B., Mungai, M., Sweetwyne, M. T., Koh, H.-J., Zaganjor, E., Damo, S. M., Gaddy, J. A., Williams, C., McReynolds, M., Marshall, A. G., Hinton, A.. 2023-09-26. Quantitative Assessment of Morphological Changes in Lipid Droplets and Lipid-Mito Interactions with Aging in Brown Adipose. https://doi.org/10.1101/2023.09.23.559135

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