Multi-modal profiling of biostabilized human skin modules reveals a coordinated ecosystem response to injected mRNA-1273 COVID-19 vaccine.

Scholaert, M.; Peries, M.; Braun, E.; Martin, J.; Serhan, N.; Loste, A.; Bruner, A.; Basso, L.; Chaput, B.; Merle, E.; Descargues, P.; Pages, E.; Gaudenzio, N.

doi:10.1101/2023.09.22.558940

bioRxiv · 10.1101/2023.09.22.558940

Multi-modal profiling of biostabilized human skin modules reveals a coordinated ecosystem response to injected mRNA-1273 COVID-19 vaccine.

Scholaert, M.·Peries, M.·Braun, E.·Martin, J.·Serhan, N.·Loste, A.·Bruner, A.·Basso, L.·Chaput, B.·Merle, E.·Descargues, P.·Pages, E.·Gaudenzio, N.

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Abstract

The field of vaccination is witnessing a remarkable surge in the development of innovative strategies. There is a need to develop technological platforms capable of generating human data prior to progressing to clinical trials. Here we introduce VaxSkin, a flexible solution designed for the comprehensive monitoring of the natural human skin ecosystems response to vaccines over time. Based on bioengineering to repurpose surgical resections, it allows a comprehensive analysis of the response to vaccines at both organ and single-cell levels. Upon injection of the mRNA-1273 COVID-19 vaccine, we characterized precise sequential molecular events triggered upon detection of the exogenous substance. We also found that the vaccine consistently targets DC/macrophages and mast cells, regardless of the administration route, while promoting specific cell-cell communications in surrounding immune cell subsets. Given its direct translational relevance, VaxSkin provides a multiscale vision of skin vaccination that could pave the way toward the development of new vaccination development strategies.

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Scholaert, M., Peries, M., Braun, E., Martin, J., Serhan, N., Loste, A., Bruner, A., Basso, L., Chaput, B., Merle, E., Descargues, P., Pages, E., Gaudenzio, N.. 2023-09-25. Multi-modal profiling of biostabilized human skin modules reveals a coordinated ecosystem response to injected mRNA-1273 COVID-19 vaccine.. https://doi.org/10.1101/2023.09.22.558940

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