An Integrated Comparative Genomics, Subtractive Proteomics and Immunoinformatics Framework for the Rational Design of a Pan-Salmonella Multi-Epitope Vaccine

Bhattacharjee, A.; Hosen, M. R.; Lamisa, A. B.; Ahammad, I.; Chowdhury, Z. M.; Jamal, T. B.; Sohag, M. M. H.; Hossain, M. U.; Das, K. C.; Keya, C. A.; Salimullah, M.

doi:10.1101/2023.09.21.558749

bioRxiv · 10.1101/2023.09.21.558749

An Integrated Comparative Genomics, Subtractive Proteomics and Immunoinformatics Framework for the Rational Design of a Pan-Salmonella Multi-Epitope Vaccine

Bhattacharjee, A.·Hosen, M. R.·Lamisa, A. B.·Ahammad, I.·Chowdhury, Z. M.·Jamal, T. B.·Sohag, M. M. H.·Hossain, M. U.·Das, K. C.·Keya, C. A.·Salimullah, M.

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Abstract

Salmonella infections are a global public health issue due to the high cost of illness surveillance, prevention, and treatment. In this study, we explored the core proteome in Salmonella to design a multi-epitope vaccine through Subtractive Proteomics and immunoinformatics approaches. A total of 2395 core proteins presents in 30 different strains of Salmonella (reference strain-NZ CP014051) were curated. Utilizing the subtractive proteomics approach on the Salmonella core proteome, Curlin major subunit A (CsgA) was selected as the vaccine candidate. csgA is a conserved gene that is related with biofilm formation. Immunodominant B and T cell epitopes from CsgA were predicted using numerous immunoinformatics tools. T lymphocyte epitopes had adequate population coverage and their corresponding MHC alleles showed significant binding scores after peptide-protein based molecular docking. Afterward, a multiepitope vaccine was constructed with peptide linkers and Human Beta Defensin-2 (as an adjuvant). The vaccine was found to be highly antigenic, non-toxic, non-allergic, and had physicochemical properties. Additionally, Molecular Dynamics Simulation and Immune Simulation demonstrated that the vaccine can bind with Toll Like Receptor 4 and elicit robust immune response. Using in vitro, in vivo, and clinical trials, our results would yield a Pan-Salmonella vaccine that will provide protection against various Salmonella species.

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Bhattacharjee, A., Hosen, M. R., Lamisa, A. B., Ahammad, I., Chowdhury, Z. M., Jamal, T. B., Sohag, M. M. H., Hossain, M. U., Das, K. C., Keya, C. A., Salimullah, M.. 2023-09-23. An Integrated Comparative Genomics, Subtractive Proteomics and Immunoinformatics Framework for the Rational Design of a Pan-Salmonella Multi-Epitope Vaccine. https://doi.org/10.1101/2023.09.21.558749

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