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bioRxiv · 10.1101/2023.09.18.558240

Probing Molecular Diversity and Ultrastructure of Brain Cells with Fluorescent Aptamers

Lu, X.·Wu, Y.·Li, P.·Fang, T.·Schalek, R. L.·Su, Y.·Carter, J. D.·Gupta, S.·Jain, V.·Janjic, N.·Lichtman, J. W.
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Abstract

Detergent-free immunolabeling has been proven feasible for correlated light and electron microscopy, but its application is restricted by the availability of suitable affinity reagents. Here we introduce CAptVE, a method using slow off-rate modified aptamers for cell fluorescence labeling on ultrastructurally reconstructable electron micrographs. CAptVE provides labeling for a wide range of biomarkers, offering a pathway to integrate molecular analysis into recent approaches to delineate neural circuits via connectomics.

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Lu, X., Wu, Y., Li, P., Fang, T., Schalek, R. L., Su, Y., Carter, J. D., Gupta, S., Jain, V., Janjic, N., Lichtman, J. W.. 2023-09-18. Probing Molecular Diversity and Ultrastructure of Brain Cells with Fluorescent Aptamers. https://doi.org/10.1101/2023.09.18.558240

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