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bioRxiv · 10.1101/2023.09.13.557582

Attenuated incubation of ethanol-induced conditioned taste aversion in a model of dependence

Ramirez, L. A.·Przybysz, K. R.·Pitock, J. R.·Starr, E. M.·Yang, H.·Glover, E. J.
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Abstract

RationalePreclinical studies report attenuated ethanol-induced conditioned taste aversion (CTA) following chronic ethanol exposure, suggesting that tolerance develops to the aversive properties of ethanol. However, these studies are confounded by pre-exposure to the unconditioned stimulus (US; ethanol), which is well known to hinder conditioning. ObjectivesThis study was designed to determine whether chronic ethanol exposure produces tolerance to the aversive properties of ethanol in the absence of a US pre-exposure confound. MethodsCTA was performed in adult male and female Long-Evans rats by pairing 0.1% ingested saccharin with an intraperitoneal injection of ethanol (1.5 or 2.0 g/kg) or saline. Rats were then rendered ethanol dependent using chronic intermittent ethanol (CIE) vapor exposure. Controls were exposed to room air (AIR). The effect of chronic ethanol on CTA expression and reconditioning were examined following vapor exposure. ResultsPrior to vapor exposure, both sexes developed CTA to a comparable degree with 2.0 g/kg producing greater CTA than 1.5 g/kg ethanol. Following vapor exposure, AIR controls exhibited an increase in CTA magnitude compared to pre-vapor levels. This effect was absent in CIE-exposed rats. These group differences were eliminated upon re-conditioning after vapor exposure. ConclusionsThese data suggest that chronic ethanol does not facilitate tolerance to the aversive properties of ethanol but rather, attenuates incubation of ethanol-induced CTA. Loss of CTA incubation suggests that CIE exposure disrupts circuits encoding aversion.

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Ramirez, L. A., Przybysz, K. R., Pitock, J. R., Starr, E. M., Yang, H., Glover, E. J.. 2023-09-14. Attenuated incubation of ethanol-induced conditioned taste aversion in a model of dependence. https://doi.org/10.1101/2023.09.13.557582

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