Of mice and men: Increased dendritic complexity gives rise to unique human networks

Kanari, L.; Shi, Y.; Arnaudon, A.; Barros Zulaica, N.; Benavides-Piccione, R.; Coggan, J. S.; DeFelipe, J. D.; Hess, K.; Mansvelder, H.; Mertens, E. J.; Segev, I.; Markram, H.; de Kock, C. P.

doi:10.1101/2023.09.11.557170

bioRxiv · 10.1101/2023.09.11.557170

Of mice and men: Increased dendritic complexity gives rise to unique human networks

Kanari, L.·Shi, Y.·Arnaudon, A.·Barros Zulaica, N.·Benavides-Piccione, R.·Coggan, J. S.·DeFelipe, J. D.·Hess, K.·Mansvelder, H.·Mertens, E. J.·Segev, I.·Markram, H.·de Kock, C. P.

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Abstract

The organizational principles that distinguish the human brain from other species have been a long-standing enigma in neuroscience. Focusing on the uniquely evolved human cortical layers 2 and 3, we computationally reconstruct the cortical architecture for mice and humans. We show that human pyramidal cells form highly complex networks, demonstrated by the increased number and simplex dimension compared to mice. This is surprising because human pyramidal cells are much sparser in the cortex. We show that the number and size of neurons fail to account for this increased network complexity, suggesting that another morphological property is a key determinant of network connectivity. Topological comparison of dendritic structure reveals much higher perisomatic (basal and oblique) branching density in human pyramidal cells. Using topological tools we quantitatively show that this neuronal structural property directly impacts network complexity, including the formation of a rich subnetwork structure. We conclude that greater dendritic complexity, a defining attribute of human L2 and 3 neurons, may provide the human cortex with enhanced computational capacity and cognitive flexibility. Graphical abstract O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=100 SRC="FIGDIR/small/557170v3_ufig1.gif" ALT="Figure 1"> View larger version (22K): org.highwire.dtl.DTLVardef@c0adadorg.highwire.dtl.DTLVardef@1ca4aaeorg.highwire.dtl.DTLVardef@1ecf1b5org.highwire.dtl.DTLVardef@f0d44d_HPS_FORMAT_FIGEXP M_FIG C_FIG A. A multiscale analysis was performed to compare the mouse and human brains: from the anatomical properties of brain regions to the morphological details of single neurons. B. Human circuits are larger than mice in terms of size and number of neurons, but present decreased neuron density, resulting in increased distances between neurons, particularly among pyramidal cells. C. Greater network complexity emerges within the human brain. Network complexity is defined by larger groups of neurons forming complex interconnections throughout the network. D. The topological analysis of layer 2/3 pyramidal cells in the temporal cortex reveals an intriguing difference: human neurons exhibit a significantly larger number of dendritic branches, especially near the cell body compared to mice. This phenomenon is termed "higher topological complexity" in dendrites. Our findings suggest that dendritic complexity wields a more substantial influence on network complexity than neuron density.

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Kanari, L., Shi, Y., Arnaudon, A., Barros Zulaica, N., Benavides-Piccione, R., Coggan, J. S., DeFelipe, J. D., Hess, K., Mansvelder, H., Mertens, E. J., Segev, I., Markram, H., de Kock, C. P.. 2023-09-12. Of mice and men: Increased dendritic complexity gives rise to unique human networks. https://doi.org/10.1101/2023.09.11.557170

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