Droplet based low input proteomic platform for rare cell populations

Waas, M.; Khoo, A.; Tharmapalan, P.; McCloskey, C. W.; Govindarajan, M.; Zhang, B.; Khan, S.; Waterhouse, P.; Khokha, R.; Kislinger, T.

doi:10.1101/2023.09.11.557098

bioRxiv · 10.1101/2023.09.11.557098

Droplet based low input proteomic platform for rare cell populations

Waas, M.·Khoo, A.·Tharmapalan, P.·McCloskey, C. W.·Govindarajan, M.·Zhang, B.·Khan, S.·Waterhouse, P.·Khokha, R.·Kislinger, T.

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Abstract

Deep proteomic profiling of rare cell populations has been constrained by sample input requirements. Here, we present DROPPS, an accessible low-input platform that generates high-fidelity proteomic profiles of 100 - 2,500 cells. By applying DROPPS within the mammary epithelium, we elucidated the connection between mitochondrial activity and clonogenicity, discovering and validating CD36 as a marker of progenitor capacity in the basal cell compartment. We anticipate DROPPS will accelerate biology-driven proteomic research for a multitude of rare cell populations.

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Waas, M., Khoo, A., Tharmapalan, P., McCloskey, C. W., Govindarajan, M., Zhang, B., Khan, S., Waterhouse, P., Khokha, R., Kislinger, T.. 2023-09-11. Droplet based low input proteomic platform for rare cell populations. https://doi.org/10.1101/2023.09.11.557098

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