Multivariate comparison of taxonomic, chemical and technical data from 80 full-scale an-aerobic digester-related systems

Otto, P.; Puchol-Royo, R.; Ortega-Legarreta, A.; Tanner, K.; Tideman, J.; de Vries, S.-J.; Pascual, J.; Porcar, M.; Latorre-Perez, A.; Abendroth, C.

doi:10.1101/2023.09.08.556802

bioRxiv · 10.1101/2023.09.08.556802

Multivariate comparison of taxonomic, chemical and technical data from 80 full-scale an-aerobic digester-related systems

Otto, P.·Puchol-Royo, R.·Ortega-Legarreta, A.·Tanner, K.·Tideman, J.·de Vries, S.-J.·Pascual, J.·Porcar, M.·Latorre-Perez, A.·Abendroth, C.

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Abstract

This study represents one of the most comprehensive characterisations of the anaerobic digestion (AD) microbiome with 80 samples from 45 different large-scale reactors in three coun-tries. Technical, chemical and taxonomic data was thoroughly collected, analyzed and correlated to identify the main drivers of AD processes. Our results showed that MBA03, Proteiniphilum, a member of Dethiobacteraceae, and Caldicoprobacter were present in all the samples, while Meth-anosarcina was the most abundant and prevalent archaea. Two distinct bacterial clusters were iden-tified by correlating microbial abundances. One was correlated with hydrogenotrophic and the other with acetoclastic methanogenesis. Organic acids, ammonia, nitrogen, COD and the trace el-ements Fe, Mo, and the macro nutrient P had the greatest impact on AD microbiomes. Temperature, reactor type and substrate also influenced the formation of a specialized microbial community. Overall, this work sheds light on the microbial key players involved in AD and evaluates how they are affected by technical and chemical parameters. HighlightsO_LIGeneration of a holistic dataset of chemical, taxonomic and technical parameters of 80 large-scale anaerobic digestion systems. C_LIO_LIIdentification of a core microbiome comprising MBA03, Proteiniphilum, an uncultured or-ganism from the Dethiobacteraceae family, and the Caldicoprobacter family. C_LIO_LIIdentification of the main influencing parameters that determine the occurrence and non-occurrence of specific genera. C_LIO_LICorrelation of bacterial taxa with hydrogenotrophic and/or acetoclastic archaea. C_LI

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Otto, P., Puchol-Royo, R., Ortega-Legarreta, A., Tanner, K., Tideman, J., de Vries, S.-J., Pascual, J., Porcar, M., Latorre-Perez, A., Abendroth, C.. 2023-09-08. Multivariate comparison of taxonomic, chemical and technical data from 80 full-scale an-aerobic digester-related systems. https://doi.org/10.1101/2023.09.08.556802

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