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bioRxiv · 10.1101/2023.09.07.556724

Seeding Activity of Skin Misfolded Tau as a Novel Biomarker for Tauopathies

Wang, Z.·Wu, L.·Gerasimenko, M.·Gilliland, T.·Shah, Z. S. A.·Gunzler, S. A.·Donadio, V.·Liguori, R.·Xu, B.·Zou, W.
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Abstract

Tauopathies are a group of age-related neurodegenerative diseases with a molecular hallmark of the prion-like propagation and accumulation of pathologically phosphorylated tau protein in the brain. They include Alzheimers disease (AD), progressive supranuclear palsy (PSP), corticobasal degeneration (CBD), and Picks disease (PiD). Currently, in the peripheral tissues and body fluids there are no reliable diagnostic biomarkers available that are able to directly reflect the capability of propagation and spreading of the misfolded tau aggregates. Here, we revealed significantly increased amounts of phosphorylated tau in the skin of AD patients compared to those in other tauopathies and normal controls. Moreover, the seed-amplification assay (SAA) by the ultrasensitive real-time quaking-induced conversion (RT-QuIC) displayed that the prion-like seeding activity of pathological tau in the skin of cadavers with neuropathologically confirmed tauopathies including AD, PSP, CBD, PiD was dramatically higher than that in normal controls, yielding 75-80% sensitivity and 95-100% specificity, respectively, depending on different tau substrates used. The increased tau-seeding activity was also observed in biopsy skin samples from living AD and PSP patients. Moreover, analysis of the end products of skin-tau SAA confirmed that the increased seeding activity is accompanied with formation of tau aggregates that are of different physicochemical properties determined by the different tau-substrates used. Our study provides proof-of-concept that the skin tau-SAA can differentiate tauopathies from normal controls, suggesting that the seeding activity of the skin misfolded tau can serve as an accurate diagnostic biomarker of tauopathies.

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Wang, Z., Wu, L., Gerasimenko, M., Gilliland, T., Shah, Z. S. A., Gunzler, S. A., Donadio, V., Liguori, R., Xu, B., Zou, W.. 2023-09-09. Seeding Activity of Skin Misfolded Tau as a Novel Biomarker for Tauopathies. https://doi.org/10.1101/2023.09.07.556724

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