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bioRxiv · 10.1101/2023.09.03.556015

Propagation of human respiratory syncytial virus in cells derived from the black flying fox (Pteropus alecto).

sugrue, r. J.·Tan, J. B.-H.·Tan, T. J. C.
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Abstract

The propagation of human respiratory syncytial virus (hRSV) was evaluated in the Pteropus alecto kidney (PaKi) cell line. At 20 hrs post-infection, immunoblotting of hRSV-infected PaKi cell lysates with anti-G, anti-N, anti-P and anti-M2-1 indicated expression of the respective virus proteins of the correct size. The hRSV-infected PaKi cell were also stained using anti-F, anti-G, anti-N, anti-P and anti-M2-1 and imaged using immunofluorescence microscopy, which confirmed high levels of virus infection, and the presence of numerous virus filaments and virus-induced inclusion bodies. PaKi cell monolayers also supported multiple cycle infection when hRSV was used to infect PaKi cells using a low multiplicity of infection. These data indicate that prior adaptation of hRSV was not required for its propagation in the PaKi cell line, and suggests that PaKi cell line is a suitable cell model system with which to examine virus-host interactions involving RSV infection in fruit bats.

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sugrue, r. J., Tan, J. B.-H., Tan, T. J. C.. 2023-09-03. Propagation of human respiratory syncytial virus in cells derived from the black flying fox (Pteropus alecto).. https://doi.org/10.1101/2023.09.03.556015

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