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bioRxiv · 10.1101/2023.09.01.555863

Single-B cell analysis correlates high-lactate secretion with stress and increased apoptosis

Abstract

While cellular metabolism was proposed to be a driving factor of the activation and differentiation of B cells and the function of the resulting antibody-secreting cells (ASCs), the study of correlations between cellular metabolism and functionalities has been difficult due to the absence of technologies enabling the parallel measurement. Herein, we performed single-cell transcriptomics and introduced a direct concurrent functional and metabolic flux quantitation of individual murine B cells. Our transcriptomic data identified lactate metabolism as dynamic in ASCs, but antibody secretion did not correlate with lactate secretion rates (LSRs). Instead, our study of all splenic B cells during an immune response linked increased lactate metabolism with acidic intracellular pH and the upregulation of apoptosis. T cell-dependent responses increased LSRs, and added TLR4 agonists affected the magnitude and boosted LSRhigh B cells in vivo, while resulting in only a few immunoglobulin-G secreting cells (IgG-SCs). Therefore, our observations indicated that LSRhigh cells were not differentiating into IgG-SCs, and were rather removed due to apoptosis.

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BibTeXRIS

Bucheli, O. T. M., Rodrigues, D., Portmann, K., Linder, A., Thoma, M., Halin, C., Eyer, K.. 2023-09-03. Single-B cell analysis correlates high-lactate secretion with stress and increased apoptosis. https://doi.org/10.1101/2023.09.01.555863

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