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bioRxiv · 10.1101/2023.09.01.555727

Respective contribution of Appl to mushroom body axon growth and long-term memory in Drosophila

Marquilly, C.·Boulanger, A.·Busto, G. U.·Pasquer, L.·Zinzen, R. P.·Hassan, B. A.·Fradkin, L. G.·Preat, T.·Dura, J.-M.
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Abstract

The Amyloid Precursor Protein (APP) is associated with Alzheimers disease. Appl is the single Drosophila APP ortholog and is expressed in all neurons throughout development. Appl was previously shown to cell-autonomously modulate axon outgrowth in the mushroom bodies (MBs), the fly olfactory memory center. However, we found that Appld, the only reported null allele, affects the normal function of vnd, the gene just proximal to Appl. To decipher developmental defects specifically due to a loss of only Appl function, we generated a precise Appl null allele (ApplC2.1) by CRISPR/Cas9 genomic engineering. With ApplC2.1, we confirmed the partial requirement for Appl in MB axon outgrowth. We also produced new CRISPR vnd alleles removing either vnd-B or vnd-A function. We report here that vnd is also required for MB {beta}-branch axon outgrowth and to a much greater extent than Appl itself. Moreover, vnd is expressed in neurons close to, but not within, the MB during development and is required non-cell-autonomously for MB axon outgrowth

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Marquilly, C., Boulanger, A., Busto, G. U., Pasquer, L., Zinzen, R. P., Hassan, B. A., Fradkin, L. G., Preat, T., Dura, J.-M.. 2023-09-03. Respective contribution of Appl to mushroom body axon growth and long-term memory in Drosophila. https://doi.org/10.1101/2023.09.01.555727

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