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bioRxiv · 10.1101/2023.08.31.555736

Is the neuropeptide PEN a ligand of GPR83?

Giesecke, Y.·Asimi, V.·Stulberg, V.·Kleinau, G.·Scheerer, P.·Koksch, B.·Groetzinger, C.
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Abstract

G protein-coupled receptor 83 (GPR83) is a class A G protein-coupled receptor with predominant expression in the cerebellum and proposed function in the regulation of food intake and in anxiety-like behavior. The neuropeptide PEN has been suggested as a specific GPR83 ligand. However, conflicting reports exist about whether PEN is indeed able to bind and activate GPR83. This study was initiated to evaluate PEN as a potential ligand of GPR83. Employing several second-messenger and other GPCR activation assays as well as a radioligand binding assay, and using multiple GPR83 plasmids and PEN peptides from different sources, no experimental evidence was found to support a role of PEN as a GPR83 ligand.

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Giesecke, Y., Asimi, V., Stulberg, V., Kleinau, G., Scheerer, P., Koksch, B., Groetzinger, C.. 2023-09-03. Is the neuropeptide PEN a ligand of GPR83?. https://doi.org/10.1101/2023.08.31.555736

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