Tracer-based lipidomics identifies novel disease-specific biomarkers in mitochondrial β-oxidation disorders

Schwantje, M.; Mosegaard, S.; Knottnerus, S. J. G.; van Klinken, J. B.; Wanders, R. J.; van Lenthe, H.; Hermans, J.; IJlst, L.; Denis, S. W.; Jaspers, Y. R. J.; Fuchs, S. A.; Houtkooper, R.; Ferdinandusse, S.; Vaz, F. M.

doi:10.1101/2023.08.31.555571

bioRxiv · 10.1101/2023.08.31.555571

Tracer-based lipidomics identifies novel disease-specific biomarkers in mitochondrial β-oxidation disorders

Schwantje, M.·Mosegaard, S.·Knottnerus, S. J. G.·van Klinken, J. B.·Wanders, R. J.·van Lenthe, H.·Hermans, J.·IJlst, L.·Denis, S. W.·Jaspers, Y. R. J.·Fuchs, S. A.·Houtkooper, R.·Ferdinandusse, S.·Vaz, F. M.

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Abstract

Carnitine derivatives of disease-specific acyl-CoAs are the diagnostic hallmark for long-chain fatty acid oxidation disorders (lcFAOD), including carnitine shuttle deficiencies, very-long-chain acyl-CoA dehydrogenase deficiency (VLCADD), long-chain 3-hydroxyacyl-CoA dehydrogenase deficiency (LCHADD) and mitochondrial trifunctional protein deficiency (MPTD). The exact consequence of accumulating lcFAO-intermediates and possible influence on cellular lipid homeostasis are, however, still unknown. To investigate the fate and cellular effects of the accumulating lcFAO-intermediates and to explore new disease markers, we used tracer-based lipidomics with deuterium-labeled oleic acid (D9-C18:1) in lcFAOD patient-derived fibroblasts. In line with previous studies, we observed a trend towards neutral lipid accumulation in lcFAOD. In addition, we detected a direct connection between the chain length and patterns of (un)saturation of accumulating acylcarnitines and the various enzyme deficiencies. Our results also identified two new candidate disease markers. Lysophosphatidylcholine(14:1) (LPC(14:1)) was specifically increased in severe VLCADD compared to mild VLCADD and control samples. This was confirmed in plasma samples showing an inverse correlation with enzyme activity, which was better than the classic diagnostic marker C14:1-carnitine. The second biomarker is an unknown lipid class, which we identified as S-(3-hydroxyacyl)cysteamines. These are hypothesized to be degradation products of the CoA moiety of accumulating 3-hydroxyacyl-CoAs. S-(3-hydroxyacyl)cysteamines were significantly increased in LCHADD compared to controls and other lcFAOD, including MTPD. Our findings suggest extensive alternative lipid metabolism in lcFAOD and confirm that lcFAOD accumulate neutral lipid species. In addition, we present two new disease markers for VLCADD and LCHADD, that may have significant relevance for disease diagnosis, prognosis, and monitoring.

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Schwantje, M., Mosegaard, S., Knottnerus, S. J. G., van Klinken, J. B., Wanders, R. J., van Lenthe, H., Hermans, J., IJlst, L., Denis, S. W., Jaspers, Y. R. J., Fuchs, S. A., Houtkooper, R., Ferdinandusse, S., Vaz, F. M.. 2023-08-31. Tracer-based lipidomics identifies novel disease-specific biomarkers in mitochondrial β-oxidation disorders. https://doi.org/10.1101/2023.08.31.555571

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