Repair of CRISPR-guided RNA breaks enables site-specific RNA editing in human cells.

Nemudraia, A.; Nemudryi, A. A.; Wiedenheft, B.

doi:10.1101/2023.08.29.555404

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bioRxiv · 10.1101/2023.08.29.555404

Repair of CRISPR-guided RNA breaks enables site-specific RNA editing in human cells.

Nemudraia, A.·Nemudryi, A. A.·Wiedenheft, B.

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Abstract

Genome editing with CRISPR RNA-guided endonucleases generates DNA breaks that are resolved by cellular DNA repair machinery. However, analogous methods to manipulate RNA remain unavailable. Here, we show that site-specific RNA breaks generated with RNA-targeting CRISPR complexes are repaired in human cells, and this repair can be used for programmable deletions in human transcripts that restore gene function. Collectively, this work establishes a technology for precise RNA manipulation with potential therapeutic applications. One-Sentence SummaryCRISPR-guided RNA breaks are repaired in human cells, and this RNA repair can be used for programmable editing of human transcriptomes.

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Nemudraia, A., Nemudryi, A. A., Wiedenheft, B.. 2023-08-29. Repair of CRISPR-guided RNA breaks enables site-specific RNA editing in human cells.. https://doi.org/10.1101/2023.08.29.555404

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