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bioRxiv · 10.1101/2023.08.17.553594

Magnetic voluntary head-fixation in transgenic rats enables lifetime imaging of hippocampal neurons

Rich, P. D.·Thiberge, S. Y.·Scott, B. B.·Guo, C.·Tervo, D. G.·Brody, C.·Karpova, A. Y.·Daw, N. D.·Tank, D. W.
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Abstract

The precise neural mechanisms within the brain that contribute to the remarkable lifetime persistence of memory remain unknown. Existing techniques to record neurons in animals are either unsuitable for longitudinal recording from the same cells or make it difficult for animals to express their full naturalistic behavioral repertoire. We present a magnetic voluntary head-fixation system that provides stable optical access to the brain during complex behavior. Compared to previous systems that used mechanical restraint, there are no moving parts and animals can engage and disengage entirely at will. This system is failsafe, easy for animals to use and reliable enough to allow long-term experiments to be routinely performed. Together with a novel two-photon fluorescence collection scheme that increases two-photon signal and a transgenic rat line that stably expresses the calcium sensor GCaMP6f in dorsal CA1, we are able to track and record activity from the same hippocampal neurons, during behavior, over a large fraction of animals lives.

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Rich, P. D., Thiberge, S. Y., Scott, B. B., Guo, C., Tervo, D. G., Brody, C., Karpova, A. Y., Daw, N. D., Tank, D. W.. 2023-08-21. Magnetic voluntary head-fixation in transgenic rats enables lifetime imaging of hippocampal neurons. https://doi.org/10.1101/2023.08.17.553594

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