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bioRxiv · 10.1101/2023.08.09.552650

Unleashing the Power of NR4A1 Degradation as a Novel Strategy for Cancer Immunotherapy

Abstract

An effective cancer therapy requires both killing cancer cells and targeting tumor-promoting pathways or cell populations within the tumor microenvironment (TME). We purposely search for molecules that are critical for multiple tumor-promoting cell types and identified nuclear receptor subfamily 4 group A member 1 (NR4A1) as one such molecule. NR4A1 has been shown to promote the aggressiveness of cancer cells and maintain the immune suppressive TME. Using genetic and pharmacological approaches, we establish NR4A1 as a valid therapeutic target for cancer therapy. Importantly, we have developed the first-of-its kind proteolysis-targeting chimera (PROTAC, named NR-V04) against NR4A1. NR-V04 effectively degrades NR4A1 within hours of treatment in vitro and sustains for at least 4 days in vivo, exhibiting long-lasting NR4A1-degradation in tumors and an excellent safety profile. NR-V04 leads to robust tumor inhibition and sometimes eradication of established melanoma tumors. At the mechanistic level, we have identified an unexpected novel mechanism via significant induction of tumor-infiltrating (TI) B cells as well as an inhibition of monocytic myeloid derived suppressor cells (m-MDSC), two clinically relevant immune cell populations in human melanomas. Overall, NR-V04-mediated NR4A1 degradation holds promise for enhancing anti- cancer immune responses and offers a new avenue for treating various types of cancer.

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BibTeXRIS

Wang, L., Xiao, Y., Luo, Y., Master, R. P., Mo, J., Kim, M.-C., Liu, Y., Patel, U. M., Li, X., Shaffer, D., Guertin, K. R., Moser, E., Smalley, K., Zhou, D., Zheng, G., Zhang, W.. 2023-08-13. Unleashing the Power of NR4A1 Degradation as a Novel Strategy for Cancer Immunotherapy. https://doi.org/10.1101/2023.08.09.552650

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