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bioRxiv · 10.1101/2023.08.02.551739

Trichinella spiralis secretes stage-specific extracellular vesicles for modulating host microenvironment for parasitism.

KhueangchiangKhwang, S.·Wu, Z.·Hosoda-Yabe, R.·Yabe, T.·Okamoto, T.·Takada, C.·Okada, H.·Ogura, S.·MAEKAWA, Y.
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Abstract

Extracellular vesicles (EVs) regulate cell-to-cell communication by transferring biomolecules within individuals or between different organisms. Trichinella spiralis is thought to secrete EVs to establish parasitism, but the detailed mechanisms of EV-mediated parasitic strategies have remained unclear. We identified EVs of T. spiralis as stage-specific in adult worms in host intestine (AW-EVs) and larvae in host muscles (ML-EVs). Stage-specific EVs exhibited unique functions, such as suppressing the expression of mucin-related genes only in AW-EVs and inducing genes associated with myoblast differentiation found in nurse cell formation in ML-EVs. Both EVs also suppressed IL-6 expression. These functions corelated with the contents of each EV, as confirmed by proteomics and whole microRNA sequencing. One of the microRNAs common to both EVs, tsp-miR-1, was involved in the suppression of IL-6 expression. These results suggest that Trichinella spiralis secretes stage-specific EVs containing unique as well as common biomolecules to regulate the host microenvironment for parasitism.

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KhueangchiangKhwang, S., Wu, Z., Hosoda-Yabe, R., Yabe, T., Okamoto, T., Takada, C., Okada, H., Ogura, S., MAEKAWA, Y.. 2023-08-03. Trichinella spiralis secretes stage-specific extracellular vesicles for modulating host microenvironment for parasitism.. https://doi.org/10.1101/2023.08.02.551739

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