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bioRxiv · 10.1101/2023.07.31.551217

Sister chromatid cohesion halts DNA loop expansion

Bastie, N.·Chapard, C.·Nejmi, S.·Mboumba, H.·thierry, a.·Beckouet, F.·Koszul, R.
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Abstract

Eukaryotic genomes are folded into DNA loops mediated by structural maintenance of chromosomes (SMC) complexes such as cohesin, condensin and Smc5/6. This organization regulates different DNA-related processes along the cell cycle such as transcription, recombination, segregation and DNA repair. During G2/M stages, SMC-mediated DNA loops coexist with cohesin complexes involved in sister chromatid cohesion (SCC). However, the articulation between the establishment of SCC and the formation of SMC-mediated DNA loops along the chromatin remains unknown. Here we show that SCC is indeed a barrier to cohesin-mediated DNA loop expansion in G2.

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Bastie, N., Chapard, C., Nejmi, S., Mboumba, H., thierry, a., Beckouet, F., Koszul, R.. 2023-07-31. Sister chromatid cohesion halts DNA loop expansion. https://doi.org/10.1101/2023.07.31.551217

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