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bioRxiv · 10.1101/2023.07.26.550516

CytoPb: Segmentation-free and threshold-free analysis of highly multiplexed images of biological tissue

Barber, P. R.·Dolcetti, L.·Yeh, C.-A.·Ng, T.
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Abstract

We present a novel method and code, CytoPb, for the analysis of multiplexed images that is segmentation-free and threshold-free and produces results that correlate well with existing methods but is faster to run, needs minimal training and removes subjectivity associated with the validation of single-cell segmentation and thresholding. In three data sets cell abundance measures correlate with previous methods (Pearsons coef. >0.75). Neighbourhood analysis and cell-cell proximity are possible.

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Barber, P. R., Dolcetti, L., Yeh, C.-A., Ng, T.. 2023-07-29. CytoPb: Segmentation-free and threshold-free analysis of highly multiplexed images of biological tissue. https://doi.org/10.1101/2023.07.26.550516

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