The Brain Electroencephalogram Microdisplay for Precision Neurosurgery

Tchoe, Y.; Wu, T.; U, H. S.; Roth, D. M.; Kim, D.; Lee, J.; Cleary, D. R.; Pizarro, P.; Tonsfeldt, K. J.; Lee, K.; Chen, P. C.; Bourhis, A. M.; Galton, I.; Coughlin, B.; Yang, J. C.; Paulk, A. C.; Halgren, E.; Cash, S. S.; Dayeh, S. A.

doi:10.1101/2023.07.19.549735

bioRxiv · 10.1101/2023.07.19.549735

The Brain Electroencephalogram Microdisplay for Precision Neurosurgery

Tchoe, Y.·Wu, T.·U, H. S.·Roth, D. M.·Kim, D.·Lee, J.·Cleary, D. R.·Pizarro, P.·Tonsfeldt, K. J.·Lee, K.·Chen, P. C.·Bourhis, A. M.·Galton, I.·Coughlin, B.·Yang, J. C.·Paulk, A. C.·Halgren, E.·Cash, S. S.·Dayeh, S. A.

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Abstract

Brain surgeries are among the most delicate clinical procedures and must be performed with the most technologically robust and advanced tools. When such surgical procedures are performed in functionally critical regions of the brain, functional mapping is applied as a standard practice that involves direct coordinated interactions between the neurosurgeon and the clinical neurology electrophysiology team. However, information flow during these interactions is commonly verbal as well as time consuming which in turn increases the duration and cost of the surgery, possibly compromising the patient outcomes. Additionally, the grids that measure brain activity and identify the boundaries of pathological versus functional brain regions suffer from low resolution (3-10 mm contact to contact spacing) with limited conformity to the brain surface. Here, we introduce a brain intracranial electroencephalogram microdisplay (Brain-iEEG-microdisplay) which conforms to the brain to measure the brain activity and display changes in near real-time (40 Hz refresh rate) on the surface of the brain in the surgical field. We used scalable engineered gallium nitride (GaN) substrates with 6" diameter to fabricate, encapsulate, and release free-standing arrays of up to 2048 GaN light emitting diodes (LEDs) in polyimide substrates. We then laminated the LED arrays on the back of micro-electrocorticography (ECoG) platinum nanorod grids (PtNRGrids) and developed hardware and software to perform near real-time intracranial EEG analysis and activation of light patterns that correspond to specific cortical activities. Using the Brain-iEEG-microdisplay, we precisely ideFSntified and displayed important cortical landmarks and pharmacologically induced pathological activities. In the rat model, we identified and displayed individual cortical columns corresponding to individual whiskers and the near real-time evolution of epileptic discharges. In the pig animal model, we demonstrated near real-time mapping and display of cortical functional boundaries using somatosensory evoked potentials (SSEP) and display of responses to direct electrical stimulation (DES) from the surface or within the brain tissue. Using a dual-color Brain-iEEG-microdisplay, we demonstrated co-registration of the functional cortical boundaries with one color and displayed the evolution of electrical potentials associated with epileptiform activity with another color. The Brain-iEEG-microdisplay holds the promise of increasing the efficiency of diagnosis and possibly surgical treatment, thereby reducing the cost and improving patient outcomes which would mark a major advancement in neurosurgery. These advances can also be translated to broader applications in neuro-oncology and neurophysiology. One Sentence SummaryA brain intracranial electroencephalogram microdisplay (Brain-iEEG-microdisplay) measures and displays real-time brain activity in the surgical field.

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Tchoe, Y., Wu, T., U, H. S., Roth, D. M., Kim, D., Lee, J., Cleary, D. R., Pizarro, P., Tonsfeldt, K. J., Lee, K., Chen, P. C., Bourhis, A. M., Galton, I., Coughlin, B., Yang, J. C., Paulk, A. C., Halgren, E., Cash, S. S., Dayeh, S. A.. 2023-07-21. The Brain Electroencephalogram Microdisplay for Precision Neurosurgery. https://doi.org/10.1101/2023.07.19.549735

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