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bioRxiv · 10.1101/2023.07.12.548718

sciMET-cap: High-throughput single-cell methylation analysis with a reduced sequencing burden

Acharya, S.·Nichols, R. V.·Rylaarsdam, L. E.·O'Connell, B. L.·Braun, T. P.·Adey, A. C.
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Abstract

DNA methylation is a key component of the mammalian epigenome, playing a regulatory role in development, disease, and other processes. Robust, high-throughput single-cell DNA methylation assays are now possible (sciMET); however, the genome-wide nature of DNA methylation results in a high sequencing burden per cell. Here, we leverage target enrichment with sciMET to capture sufficient information per cell for cell type assignment using substantially fewer sequence reads (sciMET-cap). Sufficient off-target coverage further enables the production of near-complete methylomes for individual cell types. We characterize sciMET-cap on human PBMCs and brain (middle frontal gyrus).

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Acharya, S., Nichols, R. V., Rylaarsdam, L. E., O'Connell, B. L., Braun, T. P., Adey, A. C.. 2023-07-14. sciMET-cap: High-throughput single-cell methylation analysis with a reduced sequencing burden. https://doi.org/10.1101/2023.07.12.548718

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