Adaptive introgression of a visual preference gene

Rossi, M.; Hausmann, A. H.; Alcami, P.; Moest, M. H.; Wright, D. S.; Kuo, C.-Y.; Lozano, D.; Maulana, A.; Melo-Florez, L.; Rueda-Munoz, G.; McMahon, S.; Linares, M.; McMillan, W. O.; Pardo-Diaz, C.; Salazar, C.; Merrill, R. M.

doi:10.1101/2023.07.12.548653

bioRxiv · 10.1101/2023.07.12.548653

Adaptive introgression of a visual preference gene

Rossi, M.·Hausmann, A. H.·Alcami, P.·Moest, M. H.·Wright, D. S.·Kuo, C.-Y.·Lozano, D.·Maulana, A.·Melo-Florez, L.·Rueda-Munoz, G.·McMahon, S.·Linares, M.·McMillan, W. O.·Pardo-Diaz, C.·Salazar, C.·Merrill, R. M.

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Abstract

Visual preferences are important drivers of mate choice and sexual selection, but little is known of how they evolve at the genetic level. Here we take advantage of the diversity of bright warning patterns displayed by Heliconius butterflies, which are also used during mate choice. We show that two Heliconius species have evolved the same visual mating preferences for females with red patterns by exchanging genetic material through hybridization. Extensive behavioral experiments reveal that male preferences are associated with a genomic region of increased admixture between these two species. Variation in neural expression of regucalcin1, located within this introgressed region, correlates with visual preference across populations, and disruption of regucalcin1 with CRISPR/Cas9 impairs courtship towards conspecific females, proving a direct link between gene and behavior. Our results support a role for hybridization during behavioral evolution, and show how visually-guided behaviors contributing to adaptation and speciation are encoded within the genome.

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Rossi, M., Hausmann, A. H., Alcami, P., Moest, M. H., Wright, D. S., Kuo, C.-Y., Lozano, D., Maulana, A., Melo-Florez, L., Rueda-Munoz, G., McMahon, S., Linares, M., McMillan, W. O., Pardo-Diaz, C., Salazar, C., Merrill, R. M.. 2023-07-12. Adaptive introgression of a visual preference gene. https://doi.org/10.1101/2023.07.12.548653

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