Characterization and Expression Analysis of Nitrate Reductase 6-1ABD Gene in Hexaploid Bread Wheat Under Different Nitrogen Regime

Gayatri,; Ravi, M.; Chauhan, H.; Mulani, E.; Phogat, S.; Venkatesh, K.; Mandal, P. K.

doi:10.1101/2023.07.10.548320

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bioRxiv · 10.1101/2023.07.10.548320

Characterization and Expression Analysis of Nitrate Reductase 6-1ABD Gene in Hexaploid Bread Wheat Under Different Nitrogen Regime

Gayatri,·Ravi, M.·Chauhan, H.·Mulani, E.·Phogat, S.·Venkatesh, K.·Mandal, P. K.

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Abstract

Nitrate reductase (NR) is the key rate-limiting enzyme of the nitrogen (N) assimilation process in plants, which has not been characterized in bread wheat under nitrogen stress, especially with respect to their homeologues. Total 9 NRs were identified and classified into 3 groups, which showed a close relationship with different wheat ancestors. The occurrence of N-responsive cis-acting regulatory elements like MYB, MYC, G-Box and GATA-motif confirmed their N-responsiveness. Expression of all the three groups of NR under N-stress revealed NR 6-1ABD group to be the most N-responsive, which was characterized further in detail. The study was carried out in two genotypes contrasting for their N-responsiveness (HD 2967: Highly responsive to applied N, and Choti Lerma: Less responsive to applied N) selected on the basis of field evaluation. Homeologous differences within a genotype were found much more than the genotypic differences of a specific homeologue coding sequence. Among the three homeologues, though NR 6-1D homeologue was found most responsive to N-stress, the contribution was maximum for this homeologue followed by NR 6-1A and least by NR 6-1B. We found that the expression of homeologues was linked to the presence of N-responsive cis- elements. All the homeologues of NR 6-1 in Choti Lerma were found less responsive to N-stress, in comparison to HD 2967, which might also be linked to N-use efficiency. Homeologous expression of NR 6-1ABD revealed negligible contribution of B-homeologue to N-stress. Homeologous differences of NR 6-1ABD was found much more than the genotypic differences. Hence, our study on wheat NR will be helpful in manipulating the specific homeologue of the NR gene in the future.

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Gayatri,, Ravi, M., Chauhan, H., Mulani, E., Phogat, S., Venkatesh, K., Mandal, P. K.. 2023-07-11. Characterization and Expression Analysis of Nitrate Reductase 6-1ABD Gene in Hexaploid Bread Wheat Under Different Nitrogen Regime. https://doi.org/10.1101/2023.07.10.548320

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