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bioRxiv · 10.1101/2023.07.06.547926

Filter-aided extracellular vesicle enrichment (FAEVEr)

Pauwels, J.·Van de Steene, T.·Van de Velde, J.·Eyckerman, S.·Gevaert, K.
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Abstract

AbstractExtracellular vesicles (EVs), membrane-delimited nanovesicles that are secreted by cells into the extracellular environment, are gaining substantial interest due to their involvement in cellular homeostasis and their contribution to disease pathology. The latter in particular has led to an exponential increase in interest in EVs as they are considered to be circulating packages containing potential biomarkers and are also a possible biological means to deliver drugs in a cell-specific manner. However, several challenges hamper straightforward proteome analysis of EVs as they are generally low abundant and reside in complex biological matrices. These matrices typically contain abundant protein concentrations that vastly exceed those of the EV proteome. Therefore, extensive EV isolation and purification protocols are imperative and many have been developed, including (density) ultracentrifugation, size-exclusion and precipitation methods. Here, we describe filter-aided extracellular vesicle enrichment (FAEVEr) as an approach based on 300 kDa MWCO filtration that allows the processing of multiple samples in parallel within a reasonable timeframe and at moderate cost. We demonstrate that FAEVEr is capable of quantitatively retaining EV particles on filters, whilst allowing extensive washing with the mild detergent TWEEN-20 to remove interfering non-EV proteins. The retained particles are directly lysed on the filter for a complete recovery of the EV protein cargo towards proteome analysis. Here, we validate and optimize FAEVEr on recombinant EV material and apply it on conditioned medium as well as on complex serum. Our results indicate that EVs isolated from MCF7 cells cultured with or without serum have a drastic different proteome because of nutrient deprivation.

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Pauwels, J., Van de Steene, T., Van de Velde, J., Eyckerman, S., Gevaert, K.. 2023-07-07. Filter-aided extracellular vesicle enrichment (FAEVEr). https://doi.org/10.1101/2023.07.06.547926

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