Environmental enrichment promotes adaptive responding during tests of behavioral regulation in male heterogeneous stock rats

Ishiwari, K.; King, C. P.; Martin, C. D.; Tripi, J. A.; George, A. M.; Lamparelli, A. C.; Chitre, A.; Polesskaya, O.; Richards, J. B.; Solberg Woods, L. C.; Gancarz, A.; Palmer, A. A.; Dietz, D. M.; Mitchell, S. H.; Meyer, P. J.

doi:10.1101/2023.06.30.547228

bioRxiv · 10.1101/2023.06.30.547228

Environmental enrichment promotes adaptive responding during tests of behavioral regulation in male heterogeneous stock rats

Ishiwari, K.·King, C. P.·Martin, C. D.·Tripi, J. A.·George, A. M.·Lamparelli, A. C.·Chitre, A.·Polesskaya, O.·Richards, J. B.·Solberg Woods, L. C.·Gancarz, A.·Palmer, A. A.·Dietz, D. M.·Mitchell, S. H.·Meyer, P. J.

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Abstract

Organisms must regulate their behavior flexibly in the face of environmental challenges. Failure can lead to a host of maladaptive behavioral traits associated with a range of neuropsychiatric disorders, including attention deficit hyperactivity disorder, autism, and substance use disorders. This maladaptive dysregulation of behavior is influenced by genetic and environmental factors. For example, environmental enrichment produces beneficial neurobehavioral effects in animal models of such disorders. The present study determined the effects of environmental enrichment on a range of measures related to behavioral regulation using a large cohort of male, outbred heterogeneous stock (HS) rats as subjects to mimic the genetic variability found in the human population. Subjects were reared from late adolescence onwards either in pairs in standard housing with minimal enrichment (n=200) or in groups of 16 in a highly enriched environment consisting of a large multi-level cage filled with toys, running wheels, and shelters (n=64). Rats were subjected to a battery of tests, including: (i) locomotor response to novelty, (iI) light reinforcement, (iii) social reinforcement, (iv) reaction time, (v) a patch-depletion foraging test, (vi) Pavlovian conditioned approach, (vii) conditioned reinforcement, and (viii) cocaine conditioned cue preference. Results indicated that rats housed in the enriched environment were able to filter out irrelevant stimuli more effectively and thereby regulate their behavior more efficiently than standard-housing rats. The dramatic impact of environmental enrichment suggests that behavioral studies using standard housing conditions may not generalize to more complex environments that may be more ethologically relevant.

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Ishiwari, K., King, C. P., Martin, C. D., Tripi, J. A., George, A. M., Lamparelli, A. C., Chitre, A., Polesskaya, O., Richards, J. B., Solberg Woods, L. C., Gancarz, A., Palmer, A. A., Dietz, D. M., Mitchell, S. H., Meyer, P. J.. 2023-07-03. Environmental enrichment promotes adaptive responding during tests of behavioral regulation in male heterogeneous stock rats. https://doi.org/10.1101/2023.06.30.547228

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