Labile assembly of a tardigrade protein induces biostasis

Sanchez-Martinez, S.; Nguyen, K.; Biswas, S.; Nicholson, V.; Romanyuk, A. V.; Ramirez, J. F.; KC, S.; Akter, A.; Childs, C.; Usher, E. T.; Ginell, G. M.; Yu, F.; Gollub, E.; Malferrari, M.; Francia, F.; Venturoli, G.; Martin, E. W.; Caporaletti, F.; Giubertoni, G.; Woutersen, S.; Sukenik, S.; Woolfson, D.; Holehouse, A. S.; Boothby, T. C.

doi:10.1101/2023.06.30.547219

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bioRxiv · 10.1101/2023.06.30.547219

Labile assembly of a tardigrade protein induces biostasis

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Abstract

Tardigrades are microscopic animals that survive desiccation by inducing biostasis. To survive drying tardigrades rely on intrinsically disordered CAHS proteins that form gels. However, the sequence features and mechanisms underlying gel formation and the necessity of gelation for protection have not been demonstrated. Here we report a mechanism of gelation for CAHS D similar to that of intermediate filaments. We show that gelation restricts molecular motion, immobilizing and protecting labile material from the harmful effects of drying. In vivo, we observe that CAHS D forms fiber-like condensates during osmotic stress. Condensation of CAHS D improves survival of osmotically shocked cells through at least two mechanisms: reduction of cell volume change and reduction of metabolic activity. Importantly, condensation of CAHS D is reversible and metabolic rates return to control levels after CAHS condensates are resolved. This work provides insights into how tardigrades induce biostasis through the self-assembly of CAHS gels.

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Sanchez-Martinez, S., Nguyen, K., Biswas, S., Nicholson, V., Romanyuk, A. V., Ramirez, J. F., KC, S., Akter, A., Childs, C., Usher, E. T., Ginell, G. M., Yu, F., Gollub, E., Malferrari, M., Francia, F., Venturoli, G., Martin, E. W., Caporaletti, F., Giubertoni, G., Woutersen, S., Sukenik, S., Woolfson, D., Holehouse, A. S., Boothby, T. C.. 2023-07-02. Labile assembly of a tardigrade protein induces biostasis. https://doi.org/10.1101/2023.06.30.547219

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