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bioRxiv · 10.1101/2023.06.30.547178

E-PoSa: a novel and effective tool for sampling pollen directly from flowers

Pioltelli, E.·Guzzetti, L.·Tonietti, L.·Copetta, A.·Biella, P.·Campone, L.·Galimberti, A.
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Abstract

O_LIPollinator insects are declining worldwide also due to the alteration of their diet with severe implications on their health status. Pollinators diet relies mainly on flower rewards (i.e., pollen and nectar) and a precise characterization of their chemical composition is crucial in defining pollinators nutritional ecology. In this context, the pollen represents a challenging source to investigate, especially due to operative challenges during collection operations and to the small amounts produced per flower. C_LIO_LIHere, we designed and tested a novel, easy-to-assemble tool for pollen sampling: E-PoSa (Electronic Pollen Sampler), based on the use of a portable vacuum cleaner. We compared it with some of the most used sampling methods for pollen (i.e., anthers sieving and sampling of the whole anthers) by looking at the differences in their quantitative recovery and nutritional profile. Its applicability in ecological studies was also corroborated by an assessment of its recovery rate obtained from a panel of wildflowers species in an operational environment. C_LIO_LIThe data obtained showed a significantly higher pollen recovery capacity of E-PoSa compared to the conventional sieving approach and the success in retrieving enough pollen to conduct phytochemical analyses from a broad range of flower morphologies in the field. Our results also demonstrated that high purity pollen can be collected with E-PoSa and that the device does not introduce any significant variation in the nutritional analysis compared to the conventional sieving. C_LIO_LIThis new sampling approach represents a cheap and easy-to-assemble tool encouraging its future use not only in the field of pollen nutrition but also in a wide variety of other contexts related to pollination ecology. Acknowledging the potential influences of the sampling techniques and moving towards shared standardized field protocols will advance the comprehension of species interactions and foraging patterns of pollinators and their nutritional needs. C_LI

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Pioltelli, E., Guzzetti, L., Tonietti, L., Copetta, A., Biella, P., Campone, L., Galimberti, A.. 2023-07-02. E-PoSa: a novel and effective tool for sampling pollen directly from flowers. https://doi.org/10.1101/2023.06.30.547178

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