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bioRxiv · 10.1101/2023.06.26.546242

In vitro construction and long read sequencing analysis of a 24 kb long artificial DNA sequence encoding the Universal Declaration of the Rights of Man and of the Citizen.

Leblanc, J.·Boulle, O.·Roux, E.·Nicolas, J.·Lavenier, D.·Audic, Y.
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Abstract

In absence of DNA template, the ab initio production of long double-stranded DNA molecules of predefined sequences is particularly challenging. The DNA synthesis step remains a bottleneck for many applications such as functional assessment of ancestral genes, analysis of alternative splicing or DNA-based data storage. We propose in this report a fully in vitro protocol to generate very long double-stranded DNA molecule starting from commercially available short DNA blocks in less than 3 days. This innovative application of Golden Gate assembly allowed us to streamline the assembly process to produce a 24 kb long DNA molecule storing part of the Universal Declaration of Human rights and citizens. The DNA molecule produced can be readily cloned into suitable host/vector system for amplification and selection.

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Leblanc, J., Boulle, O., Roux, E., Nicolas, J., Lavenier, D., Audic, Y.. 2023-06-26. In vitro construction and long read sequencing analysis of a 24 kb long artificial DNA sequence encoding the Universal Declaration of the Rights of Man and of the Citizen.. https://doi.org/10.1101/2023.06.26.546242

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