Improving accuracy and precision of heritability estimation in twin studies: Reassessing the measurement error assumption

Abstract

IntroductionThe conventional approach to estimating heritability in twin studies implicitly assumes either the absence of measurement error or that any measurement error is incorporated into the nonshared environment component. However, this assumption can be problematic when it does not hold or when measurement error cannot be reasonably classified as part of the nonshared environment. MethodsIn this study, we demonstrate the need for improvement in the conventional structural equation modeling (SEM) used for estimating heritability when applied to trait data with measurement errors. The critical issue revolves around an assumption concerning measurement errors in twin studies. In cases where traits are measured using samples, data is aggregated during preprocessing, with only a centrality measure (e.g., mean) being used for modeling. Additionally, measurement errors resulting from sampling are assumed to be part of the nonshared environment and are thus overlooked in heritability estimation. Consequently, the presence of intra-individual variability remains concealed. Moreover, recommended sample sizes are typically based on the assumption of no measurement errors. ResultsWe argue that measurement errors in the form of intra-individual variability are an intrinsic limitation of finite sampling and should not be considered as part of the nonshared environment. Previous studies have shown that the intra-individual variability of psychometric effects is significantly larger than the inter-individual counterpart. Here, to demonstrate the appropriateness and advantages of our hierarchical linear modeling approach in heritability estimation, we utilize simulations as well as a real dataset from the ABCD (Adolescent Brain Cognitive Development) study. Moreover, we showcase the following analytical insights for data containing non-negligible measurement errors: O_LIThe conventional SEM may underestimate heritability. C_LIO_LIA hierarchical model provides a more accurate assessment of heritability. C_LIO_LILarge samples, exceeding 100 observations or thousands of twins, may be necessary to reduce imprecision. C_LI DiscussionOur study highlights the impact of measurement error on heritability estimation and introduces a hierarchical model as a more accurate alternative. These findings have significant implications for understanding individual differences and improving the design and analysis of twin studies.