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bioRxiv · 10.1101/2023.06.23.546346

Piezo1 conspires with INF2 to promote amoeboid migration in confined environments

Abstract

To invade heterogenous tissues, transformed cells may undergo a mesenchymal to amoeboid transition (MAT). However, the molecular mechanisms regulating this transition are poorly defined. In invasive melanoma cells, we demonstrate that intracellular [Ca2+] increases with the degree of confinement in a Piezo1 dependent fashion. Moreover, Piezo1/Ca2+ is found to drive amoeboid and not mesenchymal migration in confined environments. Consistent with a model in which Piezo1 senses tension at the plasma membrane, the percentage of cells using amoeboid migration is further increased in undulating microchannels. Surprisingly, amoeboid migration was not promoted by myosin light chain kinase (MLCK), which is sensitive to intracellular [Ca2+]. Instead, we report that Piezo1/Ca2+ activates inverted formin-2 (INF2) to induce widespread actin cytoskeletal remodeling. Strikingly, the activation of INF2 is found to promote de-adhesion, which in turn facilitates MAT. Using micropatterned surfaces, we demonstrate that cells require INF2 to effectively migrate in environments with challenging mechanochemical properties. Summary StatementMigrating melanoma cells are found to rely on the activation of inverted formin-2 (INF2) by Piezo1/Ca2+ for mesenchymal to amoeboid transition (MAT) in confined environments.

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BibTeXRIS

Neelakshi, K., Caruso, A. P., Prokopiou, N., Logue, J. S.. 2023-06-25. Piezo1 conspires with INF2 to promote amoeboid migration in confined environments. https://doi.org/10.1101/2023.06.23.546346

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