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bioRxiv · 10.1101/2023.06.23.546211

Parasite-Induced IFN-g Regulates Host Defense via CD115 and mTOR-Dependent Mechanism of Tissue-Resident Macrophage Death

Martin, A.·Giri, S.·Safronova, A.·Eliseeva, S.·Kwok, S.·Yarovinsky, F.
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Abstract

Host resistance to a common protozoan parasite Toxoplasma gondii relies on a coordinated immune response involving multiple cell types, including macrophages. Embryonically seeded tissue-resident macrophages (TRMs) play a critical role in maintaining tissue homeostasis but their role in parasite clearance is poorly understood. In this study, we uncovered a crucial aspect of host defense against T. gondii mediated by TRMs. Through the use of neutralizing antibodies and conditional IFN-{gamma} receptor-deficient mice, we demonstrated that IFN-{gamma} directly mediated the elimination of TRMs. Mechanistically, IFN-{gamma} stimulation rendered macrophages unresponsive to macrophage colony-stimulating factor (M-CSF) and inactivated mTOR signaling by causing the shedding of CD115 (CSFR1), the receptor for M-CSF. Further experiments revealed the essential role of macrophage IFN-{gamma} responsiveness in host resistance to T. gondii. The elimination of peritoneal TRMs emerged as a host defense mechanism aimed at limiting the parasites reservoir. The identified mechanism, involving IFN-{gamma}-induced CD115 and mTOR-dependent cell death, provides insights into the adaptation of macrophage subsets during infection and highlights a crucial aspect of host defense against intracellular pathogens.

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Martin, A., Giri, S., Safronova, A., Eliseeva, S., Kwok, S., Yarovinsky, F.. 2023-06-25. Parasite-Induced IFN-g Regulates Host Defense via CD115 and mTOR-Dependent Mechanism of Tissue-Resident Macrophage Death. https://doi.org/10.1101/2023.06.23.546211

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