African swine fever virus I196L is a virulence determinant and its deletant induces robust protection in Domestic pig

Hu, R.; Fan, J.; Zhu, R.; Li, N.; Yang, J.; Yue, H.; Zhang, Y.; Zhou, X.; Ke, J.; Wang, Y.; Li, Q.; Qi, Y.; Miao, F.; Chen, T.; Li, M.

doi:10.1101/2023.06.22.546121

bioRxiv · 10.1101/2023.06.22.546121

African swine fever virus I196L is a virulence determinant and its deletant induces robust protection in Domestic pig

Hu, R.·Fan, J.·Zhu, R.·Li, N.·Yang, J.·Yue, H.·Zhang, Y.·Zhou, X.·Ke, J.·Wang, Y.·Li, Q.·Qi, Y.·Miao, F.·Chen, T.·Li, M.

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Abstract

The worldwide pandemic of African swine fever virus (ASFV) has a profound impact on the global pig industry. ASFV is a complex multilayered structure and the functions of unknown genes are being revealed. Here we deleted I196L from virulent ASFV SY18 with different length and obtained two recombinant viruses. The replication efficiency of the two recombinant viruses were similar but significantly lower than parental SY18. The pigs all survived the two recombinant viruses with 106.0TCID50 except one pig occurred sudden death and the suvived pigs all resisted the challenge without fever after intramuscularly injecting a lethal dose (102.0TCID50) of ASFV SY18. The recombinant viruses induced a strong anti-p54 humoral immune response. Meanwhile, the pigs also inevitably appeared moderate to high viremia throughout the observation period and presented a gradually downward trend. The results show that deleting I196L gene is a potential and effective vaccine that protects pigs from ASFV. IMPORTANCEThe worldwide outbreak of African swine fever (ASF) cannot be effectively prevented due to no availably commercial vaccine. Many different types of vaccine candidates are researched and reported, which is a hopeful trend to develop safety and efficacy vaccine. Here we report on an unknown functional gene, I196L, which affects the virulence and replication of ASFV. When I196L was deleted from ASFV SY18, the recombinant virus decreased virulence and resisted the challenge of parental strain. This is a novel, effective, potential live attenuated vaccine (LAVs) for ASF.

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Hu, R., Fan, J., Zhu, R., Li, N., Yang, J., Yue, H., Zhang, Y., Zhou, X., Ke, J., Wang, Y., Li, Q., Qi, Y., Miao, F., Chen, T., Li, M.. 2023-06-24. African swine fever virus I196L is a virulence determinant and its deletant induces robust protection in Domestic pig. https://doi.org/10.1101/2023.06.22.546121

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