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bioRxiv · 10.1101/2023.06.22.546109

Ninein domains required for its localization, association with partners dynein and ensconsin, and microtubule organization

Tillery, M. M. L.·Zheng, C.·Zheng, Y.·Megraw, T. L.
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Abstract

Ninein (Nin) is a microtubule (MT) anchor at the subdistal appendages of mother centrioles and the pericentriolar material (PCM) of centrosomes that also functions to organize microtubules at non-centrosomal microtubule-organizing centers (ncMTOCs). In humans, the NIN gene is mutated in Seckel syndrome, an inherited developmental disorder. Here we dissect the protein domains involved in Nins localization and interactions with dynein and ensconsin (ens/MAP7) and show that the association with ens cooperatively regulates microtubule assembly in Drosophila fat body cells. We define domains of Nin responsible for its localization to the ncMTOC on the fat body cell nuclear surface, localization within the nucleus, and association with Dynein light intermediate chain (Dlic) and ens, respectively. We show that Nins association with ens synergistically regulates MT assembly. Together, these findings reveal novel features of Nin function and its regulation of a ncMTOC.

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Tillery, M. M. L., Zheng, C., Zheng, Y., Megraw, T. L.. 2023-06-22. Ninein domains required for its localization, association with partners dynein and ensconsin, and microtubule organization. https://doi.org/10.1101/2023.06.22.546109

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