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bioRxiv · 10.1101/2023.06.22.546020

Thermosensitivity of translation underlies the mammalian nocturnal-diurnal switch

Beale, A. D.·Rzechorzek, N. M.·Mihut, A.·Zeng, A.·Smyllie, N. J.·Pilorz, V.·Richardson, R.·Bertlesen, M. F.·James, N. R.·Fazal, S. V.·Voysey, Z.·Pelletier, J.·Crosby, P.·Peak-Chew, S. Y.·Lancaster, M. A.·Hut, R. A.·O'Neill, J. S.
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Abstract

Early mammals were nocturnal until the Cretaceous-Paleogene extinction enabled diurnal niche expansion. Diurnality evolved multiple times independently, but the mechanisms driving this shift remain unclear. We identify a conserved cell-intrinsic signal inversion that facilitates the transition from nocturnality to diurnality. Diurnal and nocturnal mammalian cells respond oppositely to temperature and osmotic cycles, mirroring species activity patterns. Cells exhibit differential global responses to temperature changes, including the phosphoproteome and protein synthesis. mTOR signaling is identified as a central mediator of this inversion, with diurnal mammals converging on modifications to mTOR and WNK pathways during evolution. Reducing mTOR activity induces nocturnal-to-diurnal shifting at cellular, tissue, and organismal levels. Therefore, the mTOR pathway is a cellular nexus that integrates energetic state and environmental signals to determine activity niche.

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Beale, A. D., Rzechorzek, N. M., Mihut, A., Zeng, A., Smyllie, N. J., Pilorz, V., Richardson, R., Bertlesen, M. F., James, N. R., Fazal, S. V., Voysey, Z., Pelletier, J., Crosby, P., Peak-Chew, S. Y., Lancaster, M. A., Hut, R. A., O'Neill, J. S.. 2023-06-22. Thermosensitivity of translation underlies the mammalian nocturnal-diurnal switch. https://doi.org/10.1101/2023.06.22.546020

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