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bioRxiv · 10.1101/2023.06.20.545756

ShinyMultiome.UiO: An interactive open-source framework utilizing Seurat Objects for visualizing single-cell Multiomes

Akshay, A.·Sharma, A.·Eskeland, R.
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Abstract

MotivationSingle-cell genomics has been revolutionized by the advent of single-cell Multiome sequencing which allows for simultaneous profiling of chromatin accessibility and gene expression in individual nuclei. The analysis and interpretation of large and often complex scMultiome datasets requires in-depth knowledge of computational programming. ResultsWe present ShinyMultiome.UiO, a user-friendly, integrative, and opensource web-based tool that supports Seurat objects for visualization of single-cell Multiome. The ShinyMultiome.UiO facilitates interactive reporting and comprehensive characterization of cellular heterogeneity and regulatory landscapes. With ShinyMultiome.UiO, users can facilitate collaborative efforts for interpre-tation of single-cell Multiome data and make it available to the public. Availability and implementationhttps://github.com/EskelandLab/ShinyMultiomeUiO and demo server, https://cancell.medisin.uio.no/ShinyMultiome.UiO/ set up with a PBMC reference dataset. ContactAnkush.Sharma@medisin.uio.no & Ragnhild.Eskeland@medisin.uio.no

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Akshay, A., Sharma, A., Eskeland, R.. 2023-06-24. ShinyMultiome.UiO: An interactive open-source framework utilizing Seurat Objects for visualizing single-cell Multiomes. https://doi.org/10.1101/2023.06.20.545756

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