Polyploidy enhances desiccation tolerance in the grass Microchloa caffra

Abstract

Desiccation tolerance evolved recurrently across diverse plant lineages to enable survival in water limited conditions. Many resurrection plants are polyploid and several groups have hypothesized that polyploidy enabled the evolution of desiccation tolerance. However, due to the vast evolutionary divergence between resurrection plant lineages, the rarity of desiccation tolerance, and the prevalence of polyploidy in plants, this hypothesis has been difficult to test. Here, we surveyed variation in morphological, reproductive, and desiccation tolerance traits across natural populations of a single species that has differing ploidies and tested for links between polyploidy and resilience. We sampled multiple populations of the resurrection grass Microchloa caffra across an environmental gradient ranging from mesic to xeric in South Africa. We describe two distinct ecotypes of M. caffra that occupy different ends of the environmental gradient and exhibit consistent differences in ploidy, morphological, reproductive, and desiccation tolerance traits in both field and common growth conditions. Interestingly, plants with more polyploid genomes were consistently more desiccation tolerant, less reproductive, and larger than plants with smaller genomes and lower ploidy. These data suggest that polyploidy enhances desiccation tolerance and that stronger selective pressures in increasingly xeric sites may play a role in maintaining and increasing desiccation tolerance.