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bioRxiv · 10.1101/2023.06.19.545533

Meta-metabolomic Responses of River Biofilms to Cobalt Exposure and Use of Dose-response Model Trends as an Indicator of Effects

Colas, S.·Marie, B.·Milghe-Poutingon, M.·Lot, M.-C.·Boullemant, A.·Fortin, C.·Le Faucheur, S.
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Abstract

Metabolites are low molecular-weight molecules produced during cellular metabolism. The global expression of the meta-metabolome (metabolomics at the community level) could thus potentially be used to characterize the exposure of an organism or a community to a specific stressor. Here, the meta-metabolomic fingerprints of mature biofilms were examined after 1, 3 and 7 days of exposure to five concentrations of cobalt (0, 1 x 10-7, 1 x 10-6, 5 x 10-6 and 1 x 10-5 M) in aquatic microcosms. The global changes in meta-metabolomic fingerprints were in good agreement with those of the other biological parameters studied (cobalt bioaccumulation, biomass, chlorophyll content). To better understand the dose-responses of the biofilm meta-metabolome, the untargeted LC-HRMS metabolomic data were further processed using the DRomics tool to build dose-response model curves and to calculate benchmark doses (BMD). These BMDs were aggregated into an empirical cumulative density function. A trend analysis of the metabolite dose-response curves suggests the presence of a concentration range inducing defense mechanisms (CRIDeM) between 4.7 x 10-7 and 2.7 x 10-6 M, and of a concentration range inducing damage mechanisms (CRIDaM) from 2.7 x 10-6 M to the highest Co concentration. The present study demonstrates that the molecular defense and damage mechanisms can be related to contaminant concentrations and represent a promising approach for environmental risk assessment of metals. O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=94 SRC="FIGDIR/small/545533v1_ufig1.gif" ALT="Figure 1"> View larger version (37K): org.highwire.dtl.DTLVardef@179fd06org.highwire.dtl.DTLVardef@1d2cd10org.highwire.dtl.DTLVardef@9508a3org.highwire.dtl.DTLVardef@6a1818_HPS_FORMAT_FIGEXP M_FIG C_FIG SYNOPSISThis study focuses on the interpretation of the metabolite dose-response trends in river biofilms exposed to cobalt to identify concentration range inducing cellular mechanisms and improve the environmental risk assessment of metals.

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Colas, S., Marie, B., Milghe-Poutingon, M., Lot, M.-C., Boullemant, A., Fortin, C., Le Faucheur, S.. 2023-06-19. Meta-metabolomic Responses of River Biofilms to Cobalt Exposure and Use of Dose-response Model Trends as an Indicator of Effects. https://doi.org/10.1101/2023.06.19.545533

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