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bioRxiv · 10.1101/2023.06.09.544326

T- and L-type calcium channels maintain calcium oscillations in the murine zona glomerulosa

Abstract

The zona glomerulosa of the adrenal gland is responsible for the synthesis and release of the mineralocorticoid aldosterone. This steroid hormone regulates salt reabsorption in the kidney and blood pressure. The most important stimuli of aldosterone synthesis are the serum concentrations of angiotensin II and potassium. In response to these stimuli, voltage and intracellular calcium levels in the zona glomerulosa oscillate, providing the signal for aldosterone synthesis. It was proposed that the voltage-gated T-type calcium channel CaV3.2 is necessary for the generation of these oscillations. However, Cacna1h knockout mice have normal plasma aldosterone levels, suggesting additional calcium entry pathways. We used a combination of calcium imaging, patch clamp and RNA sequencing to investigate such pathways in the murine zona glomerulosa. Cacna1h-/- glomerulosa cells still showed calcium oscillations with similar concentrations as wild-type mice. No calcium channels or transporters were upregulated to compensate for the loss of CaV3.2. The calcium oscillations observed were instead dependent on L-type voltage-gated calcium channels. Furthermore, we found that L-type can also partially compensate for an acute inhibition of CaV3.2 in wild-type mice. Only inhibition of both, T- and L-type calcium channels abolished the increase of intracellular calcium caused by angiotensin II in wild-type. Our study demonstrates that T-type calcium channels are not strictly required to maintain glomerulosa calcium oscillations and aldosterone production and pharmacological inhibition of T-type channels alone will likely not significantly impact aldosterone production over time.

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BibTeXRIS

Dinh, H. A., Volkert, M., Secener, A. K., Scholl, U. I., Stölting, G.. 2023-06-11. T- and L-type calcium channels maintain calcium oscillations in the murine zona glomerulosa. https://doi.org/10.1101/2023.06.09.544326

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