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bioRxiv · 10.1101/2023.06.07.544031

Cross-reactivity of antibodies against T cell markers in the Bank vole (Myodes glareolus)

Migalska, M.·Weglarczyk, K.·Mezyk-Kopec, R.·Baliga-Klimczyk, K.·Homa, J.
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Abstract

The bank vole is a common Cricetidae rodent that is a reservoir of several zoonotic pathogens and an emerging model in eco-immunology. Here, we add to a developing immunological toolkit for this species by testing the cross-reactivity of commercially available monoclonal antibodies (mAbs) to the bank vole lymphocyte differentiation molecules and a transcription factor. We show that a combination of mAbs against CD4, CD3, and Foxp3 allows flow cytometric distinction of the main subsets of T cells: putative helper CD4+, cytotoxic CD8+ (as CD3+CD4-) and regulatory CD4+Foxp3+. We also provide a comparative analysis of amino acid sequences of CD4, CD8{beta}, CD3{varepsilon}{gamma}{delta} and Fopx3 molecules for a number of commonly studied Cricetidae rodents and discuss mAb cross-reactivity patterns reported so far in this rodent family.

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Migalska, M., Weglarczyk, K., Mezyk-Kopec, R., Baliga-Klimczyk, K., Homa, J.. 2023-06-09. Cross-reactivity of antibodies against T cell markers in the Bank vole (Myodes glareolus). https://doi.org/10.1101/2023.06.07.544031

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