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bioRxiv · 10.1101/2023.06.05.543696

Stable Structures or poly(A)-binding protein loading protect cellular and viral RNAs against ISG20-mediated decay

Abstract

ISG20 is an interferon-induced 3-to-5 RNA exonuclease that acts as a broad antiviral factor. At present, the features that expose RNA to ISG20 remain unclear, although recent studies have pointed to the modulatory role of epitranscriptomic modifications in the susceptibility of target RNAs to ISG20. These findings raise the question as to how cellular RNAs, on which these modifications are abundant, cope with ISG20. To obtain an unbiased perspective on this topic, we used RNAseq and biochemical assays to identify elements that regulate the behavior of RNAs against ISG20. The results we have obtained indicate that poly(A)-binding protein (PABP1) loading on the RNA 3 tail provides a primal protection against ISG20, easily explaining the overall protection of cellular mRNAs observed by RNAseq. The second element we uncovered is provided by terminal stem-loop RNA structures, that have been associated to ISG20 protection before, but that we re-examine here systematically to define the stabilities that tilt the balance between resistance and susceptibility to ISG20. Given that these elements are present on cellular mRNAs, but can be co-opted by viruses as well, these results shed new light on the complex interplay that regulates the susceptibility of different classes of viruses against ISG20.

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BibTeXRIS

Louvat, C., Deymier, S., Nguyen, X.-N., Labaronne, E., Noy, K., Cariou, M., Corbin, A., Mateo, M., Ricci, E. P., Fiorini, F., Cimarelli, A.. 2023-06-05. Stable Structures or poly(A)-binding protein loading protect cellular and viral RNAs against ISG20-mediated decay. https://doi.org/10.1101/2023.06.05.543696

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