Individualized network analysis reveals a link between the gut microbiome, diet intervention and Gestational Diabetes Mellitus

Abstract

Gestational Diabetes Mellitus (GDM), a serious complication during pregnancy which is defined by abnormal glucose regulation, is commonly treated by diabetic diet and lifestyle changes. While recent findings place the microbiome as a natural mediator between diet interventions and diverse disease states, its role in GDM is still unknown. Here, based on observation data from healthy pregnant control group and GDM patients, we developed a new network approach using patterns of co-abundance of microorganism to construct microbial networks that represent human-specific information about gut microbiota in different groups. By calculating network similarity in different groups, we analyze the gut microbiome from 27 GDM subjects collected before and after two weeks of diet therapy compared with 30 control subjects to identify the health condition of microbial community balance in GDM subjects. Although the microbial communities remain similar after the diet phase, we find that the structure of their inter-species co-abundance network is significantly altered, which is reflected in that the ecological balance of GDM patients was not "healthier" after the diet intervention. In addition, we devised a method for individualized network analysis of the microbiome, thereby a pattern is found that individuals with large deviations in microbial networks are usually accompanied by their abnormal glucose regulation. This approach may help the development of individualized diagnosis strategies and microbiome-based therapies in the future. Author SummaryIn this study, we aimed to investigate the role of the gut microbiome in gestational diabetes mellitus (GDM), a condition that affects pregnant women and is characterized by abnormal glucose regulation. Specifically, we asked whether and how the gut microbiome is affected by diabetic diet which is commonly used to treat GDM patients. We developed a new network approach to analyze patterns of co-abundance of microorganisms in the gut microbiota of GDM patients and healthy pregnant women. Our findings show that although the microbial communities remained similar after the diet phase, the structure of their inter-species co-abundance network was significantly altered, indicating that the ecological balance of GDM patients was not "healthier" after the diet intervention. Furthermore, we suggest that abnormal glucose regulation is associated with large network deviations, which could lead to the development of individualized microbiome-based therapies in the future. Our work highlights the importance of studying the microbiome from a network perspective to better understand the dynamic interactions among microorganisms in the community balance of the microbiome.