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bioRxiv · 10.1101/2023.05.24.542098

ClearX9-Stem™: an efficient, affordable & sustainable stem cell culture medium for biotech and cell-cultivated meat industries

Gautam, S. K.·Verma, N.·Manvati, S.·Dhar, P. K.
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Abstract

Stem cells are extraordinary cells with a unique ability of self-renewal and differentiation into various cell types such as muscle, nerve, bone, and blood cells. Historically, they have found significant applications in the biotech and pharma sectors. To grow and maintain stem cells artificially, researchers use basal media formulations supplemented with nutrients and growth factors, with Fetal bovine serum (FBS) as the key component of the culture medium. However, to maintain this supply every year, millions of pregnant cows are slaughtered for preparing FBS. The process of harvesting FBS also raises concerns about contamination with pathogens, animal proteins that may interfere with cellular behavior and ethical considerations regarding animal welfare. To overcome these limitations, here we report ClearX9-Stem - an affordable, sustainable, effective, and ethical replacement for an FBS-enriched stem cell culture medium. A specialized ClearX9-Stem cell culture medium formulation was designed to grow chicken embryonic fibroblast (SL-29) in the absence of FBS. Based on the results obtained, ClearX9-Stem is undergoing further refinement to meet the growing academic and industrial demand for serum-free culture media formulations. In the future, there is a need to customize and optimize ClearX9-Stem for the scalable growth of cells in bioreactors. HIGHLIGHTSO_LIClearX9-Stem provides good nutritional support for the growth of chicken embryonic fibroblast cells. C_LIO_LIClearX9-Stem cell growth performance is comparable to the serum-enriched culture medium C_LIO_LIClearX9-Stem maintains a healthy morphological profile of cells during division C_LIO_LIClearX9-Stem generates a stress-free environment within cells C_LIO_LIClearX9-Stem does not require animal slaughter and reduces the environmental footprint C_LIO_LIClearX9-Stem has applications in the biotechnology, pharma, and cell-cultivated meat industries C_LI

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Gautam, S. K., Verma, N., Manvati, S., Dhar, P. K.. 2023-05-25. ClearX9-Stem™: an efficient, affordable & sustainable stem cell culture medium for biotech and cell-cultivated meat industries. https://doi.org/10.1101/2023.05.24.542098

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